Journal: Discover. Oncology

Article Title: Is serum thymidine kinase 1 a prognostic biomarker in primary tumor location of colorectal carcinomas?

doi: 10.1007/s12672-023-00614-5

Figure Lengend Snippet: Characteristics of TK1-IgY-pAb. A . Example of a patient with RC (T1N2M0). a Western blot of STK1p. The serum samples, presurgery (Lines 1 and 2, duplicate), and 6 months after surgery (Line 3); Serum sample from a disease-free person (Lines 4 and 5, duplicate). b ) TK1 immunohistochemistry (IHC) staining of RC tissue postsurgery (T1N2M0). Brownish-yellow TK1 was mainly in the cytoplasm. Blue staining was used to counterstain nuclei with hematoxylin. Magnification 200 × . B . Receiver operation characteristic (ROC) analysis. The analysis was based on STK1p values of 488 CRC patients and 488 tumor-free persons

Article Snippet: Briefly, Sections. (4-μm-thick) were prepared from formalin-fixed paraffin-embedded surgical specimens from the RC patient by deparaffinization and rehydration and then incubated with TK1-IgY-pAb (1.0 μg/ml, in PBS) overnight at 4 °C followed by incubation with biotinylated donkey anti-chicken IgY antibody at room temperature for 60 min. SA-HRP (streptavidin-HRP conjugate, Invitrogen, SA10001) was added and incubated at room temperature for 90 min. Fresh diaminobenzidine (DAB) solution was used for color rendering, and the slides were lightly counterstained with hematoxylin.

Techniques: Western Blot, Immunohistochemistry, Staining

Journal: Journal of Ovarian Research

Article Title: MSCs–derived EVs protect against chemotherapy-induced ovarian toxicity: role of PI3K/AKT/mTOR axis

doi: 10.1186/s13048-024-01545-7

Figure Lengend Snippet: Effect of MSCs-EVs, rapamycin, and quercetin on ( A ) phosphorylation of mTOR, PI3K, and AKT as detected by western blot analysis, ( B - D ) Intensity of immunoreactivity of selected proteins as quantified by densitometry. Results are expressed as mean ± SEM. **significant vs. Control group at p<0.01, **** at p<0.0001, # significant vs. OF group at p<0.05, ## at p<0.01, ### at p<0.001, #### at p<0.0001, & significant vs. OF + Rapamycin group at p<0.05

Article Snippet: Next, the blots were incubated with the appropriate primary antibodies for an entire night at 4 °C, PTEN (E-AB-63495, Elabscience, USA), FOXO3 (NBP2-16521, Novus Biologicals USA), mTOR (sc-517464, Santa Cruz Biotechnology, USA), Phospho-mTOR (sc-293133, Santa Cruz Biotechnology, USA), PI3K (E-AB-64202, Elabscience, USA), Phospho-PI3K (E-AB-20966, Elabscience, USA), AKT (E-AB-15441, Elabscience, USA), Phospho-AKT (E-AB-20804, Elabscience, USA), β-actin (E-AB-20031, Elabscience, USA).

Techniques: Phospho-proteomics, Western Blot, Control